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Product Specifications
Analytical sensitivity
26.4 pg/mL
Assay range
40.96-4000 pg/mL
Sample type/volume
Fecal Extract
50 µL
Plasma
1 µL
Serum
1 µL
Supernatant
50 µL
Urine
6.25 µL
Hands-on time
1 hr 20 min
Time-to-result
2 hr 30 min
Homogenous (no wash)
No
Interassay CV
8.5%
Intraassay CV
4.2%
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
HRP-Conjugated Detection Antibody
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
HRP-Conjugated Detection Antibody
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Dry ice
Storage
-20°C
Protein name
Estrone-3-Sulfate
Species (tested)
Chemical
Assay kit format
Competitive ELISA
Detector antibody conjugate
HRP
Label or dye
HRP
About This Kit
The Human Estrone-3 Sulfate (E1S) ELISA quantitates human estrone-3 Sulfate in human serum, plasma, urine, dried fecal extracts or cell culture medium.
Principle of the method
The Estrone-3-Sulfate (E1S) Competitive ELISA research-use-only kit uses a specifically generated antibody to measure Estrone-3-Sulfate (E1S) in a variety of matrices, including serum, plasma, urine, and fecal samples. The kit will quantitatively measure E1S present in diluted buffer samples and tissue culture media samples. An E1S standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. An E1S-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to E1S to each well. After a 2-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound E1S-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
Estrone-3-sulfate (1, 3, 5(10)-Estratrien-3-ol-17-one sulfate (E1S) is synthesized in the fetal or cotyledonary portion of the placentome. Estrone sulfate, present in plasma in a higher concentration than either unconjugated estrone or estradiol in nonpregnant women and normal men, appears to originate almost entirely from a conjugation of estrone and converted estradiolin nonglandular tissues. Estrone sulfate is only slowly cleared from plasma, thus its concentration does not fluctuate markedly during the day. Estrone sulfate is quantitatively the most important circulating estrogen. Estrone-3-sulfate (E1S) is identical across all species and so this kit should measure estrone-3-sulfate from all sources.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases : C18H22O5S, Estrone sulfate
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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