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Product Specifications
Analytical sensitivity
0.94 pg/mL
Assay range
1.56-100 pg/mL
Sample type/volume
Plasma
50 µL
Serum
50 µL
Hands-on time
1 hr 20 min
Time-to-result
3 hr 30 min
Homogenous (no wash)
No
Interassay CV
<10%
Intraassay CV
<10%
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Shipping conditions
Wet ice
Storage
Refer to product documentation for component specific storage temperature.
Protein name
FT4
Protein family
Thyroid hormone
Species (tested)
All
Assay kit format
Competitive ELISA
Label or dye
HRP
About This Kit
The multispecies Free Thyroxine (fT4) ELISA quantitates fT4 in serum, plasma, and other biological fluids
Principle of the method:
The Competitive multispecies ELISA research-use-only kit is designed to quantitatively measure Free Thyroxine (fT4) independent of species. A fT4 standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to fT4 added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of fT4 in the samples is then determined by comparing the OD of the samples to the standard curve.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
Free Thyroxine (FT4) is a hormone produced by the thyroid gland and plays a crucial role in regulating metabolism, growth, and development in the body. It is transported in the bloodstream and acts on target cells by binding to thyroid hormone receptors. The structure of FT4 is characterized by a tyrosine residue linked to an iodine atom, forming the thyroid hormone. It has a molecular weight of 777.00 g/mol and a chemical formula of C15H11I4NO4. FT4 is primarily produced in the thyroid gland and is regulated by the hypothalamic-pituitary-thyroid axis. Its levels are typically measured in blood tests to diagnose thyroid disorders.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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