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Product Specifications
Analytical sensitivity
<0.5 ng/mL
Assay range
0.63-20 ng/mL
Sample type/volume
Plasma
20 µL
Serum
20 µL
Supernatant
20 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr
Homogenous (no wash)
No
Interassay CV
5%
Intraassay CV
4.8%
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Standard Dilution Buffer
Biotinylated Detection Antibody
Streptavidin-HRP
HRP Diluent
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Standard Dilution Buffer
Biotinylated Detection Antibody
Streptavidin-HRP
HRP Diluent
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
Growth Hormone
Protein subtype
Adaptor Proteins
Species (tested)
Rat
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Rat Growth Hormone ELISA Kit quantifies rat growth hormone in serum, plasma, buffered solution, or cell culture medium. The assay will recognize both the natural and recombinant growth hormone.
Principle of the method
The rat growth hormone solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
Human growth hormone (hGH) is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The GH1 gene, along with 4 additional related genes, is located at the growth hormone locus on chromosome 17. The five genes share a remarkably high degree of sequence identity. Alternative splicing results in 5 isoforms ranging from approximately 19 kDa to 24 kDa. HGH is produced by the somatotroph cells in the pituitary. It acts through binding to membrane receptors that belong to the cytokine receptor subfamily. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues. Mutations or deletions in the gene lead to growth hormone deficiency and short stature.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Gene aliases : Gh, Gh1, RNGHGP
Gene ID : (Rat) 24391
Gene symbol : Gh1
Protein Aliases : Growth hormone, Somatotropin
UniProt ID (Rat) P01244
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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