Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
The Human Agouti Related Peptide (Hu AgRP) ELISA quantitates Hu AgRP in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu AgRP.
Principle of the method
The Human AgRP solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Agouti-related protein (AgRP), also called agouti-related peptide, is a neuropeptide produced in the brain by the AgRP/NPY neuron. In humans, the agouti-related peptide is encoded by the AGRP gene. This gene encodes an antagonist of the melanocortin-3 and melanocortin-4 receptor. It appears to regulate hypothalamic control of feeding behavior via melanocortin receptor and/or intracellular calcium regulation, and thus plays a role in weight homeostasis. Mutations in this gene have been associated with late on-set obesity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Gene aliases : AGRP, AGRT, ART, ASIP2
Gene ID : (Human) 181
Gene symbol : AGRP
Protein Aliases : agouti related protein homolog, Agouti-related protein
UniProt ID (Human) O00253
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