Technical guide & protocol Tech Support

Product Specifications

Analytical sensitivity

0.4 ng/mL

Assay range

0.41-100 ng/mL

Sample type/volume

Serum
50 µL
Plasma
50 µL
Supernatant
50 µL

Hands-on time

1 hr 20 min

Time-to-result

4 hr 45 min

Homogenous (no wash)

No

Interassay CV

<12%

Intraassay CV

<10%

Instrument

Colorimetric Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

2-8°C

Protein name

CD275 (B7-H2)

Species (tested)

Human

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

Human B7-H2/ICOS Ligand quantitates human B7-H2/ICOS Ligand in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human B7-H2/ICOS Ligand.

Target information

Inducible co-stimulator ligand (ICOSL) is a specific ligand on antigen-presenting cells and cells of the peripheral tissue that binds to the inducible co-stimulator receptor (ICOS). ICOS belongs to the CD28/CD152 receptor family that regulates T-cell activation and function. ICOSL is expressed on monocytes, dendritic cells and B cells and can be induced by inflammatory stimuli in peripheral tissue. Binding to ICOSL delivers a co-stimulatory signal for T cell proliferation and cytokine secretion.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene aliases : B7-H2, B7H2, B7RP-1, B7RP1, CD275, GL50, ICOS-L, ICOSL, ICOSLG, KIAA0653, LICOS

Gene ID : (Human) 23308

Gene symbol : ICOSLG

Protein Aliases : B7 homolog 2, B7 homologue 2, B7-H2, B7-like protein Gl50, B7-related protein 1, B7RP-1, CD275, ICOS ligand, inducible T-cell co-stimulator ligand, transmembrane protein B7-H2 ICOS ligand

UniProt ID (Human) O75144

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