Technical guide & protocol Tech Support

Product Specifications

Analytical sensitivity

0.13 ng/mL

Assay range

0.31-20.0 ng/mL

Sample type/volume

Plasma
50 µL
Serum
50 µL
Supernatant
50 µL

Hands-on time

15 min

Time-to-result

3 hr 10 min

Homogenous (no wash)

No

Interassay CV

9.8%

Intraassay CV

8.2%

Instrument

Colorimetric Microplate Reader

Product size

128 Tests

Contents

Pre-coated 96 well Microplate
Biotinylated Detection Antibody
SAV-HRP
Sample Diluent, lyophilized
Standard (8-well strips x 4)
Wash Buffer Concentrate
Substrate Solution
Sample Diluent
Stop Solution
Lysis Buffer 10x
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

-20°C

Protein name

BAFF (BLyS)

Species (tested)

Human

Assay kit format

Instant ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

The Human B cell Activating Factor belonging to the TNF Family (Hu BAFF) ELISA quantitates Hu BAFF in human serum, plasma, cell culture supernatants or other body fluids. The assay will exclusively recognize both natural and recombinant Hu BAFF.

Principle of the method

The Human BAFF solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Target information

BAFF is a membrane bound cytokine expressed by B cell lineage cells. The soluble form of the cytokine binds to the receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. BAFF is a potent B cell activator, and important for the proliferation and differentiation of B cells. BAFF is also expressed by T lymphocytes and has been shown to promote T cell activation and survival. Isoforms representing alternatively spliced, and glycosylated variants have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Gene aliases : BAFF, BLYS, CD257, DTL, TALL-1, TALL1, THANK, TNFSF13B, TNFSF20, TNLG7A, UNQ401/PRO738, ZTNF4

Gene ID : (Human) 10673

Gene symbol : TNFSF13B

Protein Aliases : ApoL related ligand TALL-1, B lymphocyte stimulator, B-cell-activating factor, B-lymphocyte stimulator, BAFF, BLyS, CD257, delta BAFF, Delta4 BAFF, Dendritic cell-derived TNF-like molecule, TALL-1, TNF and ApoL-related leukocyte expressed ligand 1, TNF homolog that activates apoptosis, TNF- and APOL-related leukocyte expressed ligand 1, tumor necrosis factor (ligand) superfamily member 13b, tumor necrosis factor (ligand) superfamily, member 13b, member 20, tumor necrosis factor ligand 7A, Tumor necrosis factor ligand superfamily member 13B, tumor necrosis factor-like protein ZTNF4

UniProt ID (Human) Q9Y275

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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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