Technical guide & protocol Tech Support

Product Specifications

Analytical sensitivity

0.185 pg/mL

Assay range

0.4-25 pg/mL

Sample type/volume

Cell Lysate
50 µL
Serum
50 µL
Plasma
50 µL

Hands-on time

1 hr 20 min

Time-to-result

4 hr

Homogenous (no wash)

No

Interassay CV

4.9%

Intraassay CV

4.7%

Instrument

Colorimetric Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate
Standard (2 vials)
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
Streptavidin-HRP
Wash Buffer
Substrate Solution
Stop Solution
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

2-8°C

Protein name

Endothelin 1

Target sub-specificity

Soluble

Protein family

Vascular Protein

Species (tested)

Human

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

The Human Big ET-1 Platinum ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human Big ET-1. Cell culture supernatant, serum, and plasma (EDTA, citrate, heparin) have been tested with this assay.

Target information

The protein encoded by this gene is proteolytically processed to release a secreted peptide termed endothelin 1. This peptide is a potent vasoconstrictor and is produced by vascular endothelial cells. Endothelin 1 also can affect the central nervous system. Two transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene aliases : ARCND3, EDN1, ET1, HDLCQ7, PPET1, QME

Gene ID : (Human) 1906

Gene symbol : EDN1

Protein Aliases : EDN1, endothelin 1, Endothelin-1, ET1, HDLCQ7, PPET1, preproendothelin-1, Preproendothelin-1

UniProt ID (Human) P05305

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