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Product Specifications

Analytical sensitivity

2.60 pg/mL

Assay range

15.6-1,000 pg/mL

Sample type/volume

Plasma
10 µL
Serum
10 µL
Supernatant
10 µL

Hands-on time

15 min

Time-to-result

3 hr 30 min

Homogenous (no wash)

No

Interassay CV

3.3%

Intraassay CV

4.6%

Instrument

Colorimetric Microplate Reader

Product size

128 Tests

Contents

Pre-coated 96 well Microplate
Biotinylated Detection Antibody
SAV-HRP
Sample Diluent, lyophilized
Standard (8-well strips x 4)
Wash Buffer Concentrate
Substrate Solution
Sample Diluent
Stop Solution
Lysis Buffer 10x
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

-20°C

Protein name

CD105 (Endoglin)

Target sub-specificity

Soluble

Species (tested)

Human

Assay kit format

Instant ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

The Human Soluble CD105/Endoglin (Hu sCD105) ELISA quantitates Hu sCD105 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu sCD105.

Principle of the method

The Human sCD105 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Target information

CD105 or Endoglin is a Type I transmembrane protein, which is highly expressed on human vascular endothelial cells. It exists on an O- and N-glycosylated homodimer. Up regulation of endoglin expression has been demonstrated in tumor vasculature and proliferating cells, suggesting that it is a proliferation associated endothelial marker. CD105 binds to TGF beta 1 and 3 with high affinity but not to TGF beta 2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Gene aliases : END, ENG, HHT1, ORW1

Gene ID : (Human) 2022

Gene symbol : ENG

Protein Aliases : CD105, CD105 antigen, Endoglin

UniProt ID (Human) P17813

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