Technical guide & protocol Tech Support

Product Specifications

Analytical sensitivity

0.2 ng/mL

Assay range

0.78-50 ng/mL

Sample type/volume

Serum
50 µL
Supernatant
50 µL

Hands-on time

1 hr 20 min

Time-to-result

3 hr 10 min

Homogenous (no wash)

No

Interassay CV

9.9%

Intraassay CV

7.1%

Instrument

Colorimetric Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate
Standard
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

2-8°C

Protein name

4-1BB (CD137)

Target sub-specificity

Soluble

Species (tested)

Human

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

The Human Soluble CD137 (4-1BB) (Hu CD137) ELISA quantitates Hu CD137 in human serum or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu CD137.

Principle of the method

The Human CD137 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Target information

CD137, also known as TNFRSF9 or 4-1BB, is an inducible costimulatory molecule expressed mainly on activated T cells. Its ligand, known as 4-1BBL, is expressed on activated macrophages, mature B cells, hematopoietic stem cells, and myeloid progenitor cells. CD137 signaling leads to maintaining the survival of activated T cells and CD8+ memory T cells, and clonal expansion of T cells, but also to suppressing myelopoiesis and dendritic cell development. Triggered CD137 induces a cytokine release profile regulating peripheral monocyte survival. Soluble forms of CD137 may provide negative control mechanism for some immune responses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Gene aliases : 4-1BB, CD137, CDw137, ILA, TNFRSF9

Gene ID : (Human) 3604

Gene symbol : TNFRSF9

Protein Aliases : 4-1BB ligand receptor, CDw137, T-cell antigen 4-1BB homolog, T-cell antigen ILA, CD137, CD137 antigen, homolog of mouse 4-1BB, induced by lymphocyte activation (ILA), interleukin-activated receptor, homolog of mouse Ly63, receptor protein 4-1BB, T cell antigen ILA, Tumor necrosis factor receptor superfamily member 9, tumor necrosis factor receptor superfamily, member 9

UniProt ID (Human) Q07011

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