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Product Specifications
Analytical sensitivity
0.33 ng/mL
Assay range
1.6-100 ng/mL
Sample type/volume
Plasma
25 µL
Serum
25 µL
Supernatant
25 µL
Urine
25 µL
Hands-on time
15 min
Time-to-result
3 hr 10 min
Homogenous (no wash)
No
Interassay CV
12.9%
Intraassay CV
9.2%
Instrument
Colorimetric Microplate Reader
Product size
128 Tests
Contents
Pre-coated 96 well Microplate
Detection Antibody
Anti-IgG-HRP
Sample Diluent, lyophilized
Standard (8-well strips x 4)
Wash Buffer Concentrate
Substrate Solution
Sample Diluent
Stop Solution
Lysis Buffer 10x
Adhesive Plate Covers
Detection Antibody
Anti-IgG-HRP
Sample Diluent, lyophilized
Standard (8-well strips x 4)
Wash Buffer Concentrate
Substrate Solution
Sample Diluent
Stop Solution
Lysis Buffer 10x
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
-20°C
Protein name
CD30
Species (tested)
Human
Assay kit format
Instant ELISA Kit
Detector antibody conjugate
HRP
Label or dye
HRP
About This Kit
The Human Soluble CD30 ELISA quantitates Hu sCD30 in human serum, plasma, cell culture supernatants, urine, or other body fluids. The assay will exclusively recognize both natural and recombinant Hu sCD30.
Principle of the method
The Human sCD30 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
CD30 (Ki-1, TNF Receptor Superfamily Member 8) is a type I transmembrane glycoprotein of the TNF receptor superfamily. CD30 was originally identified as a cell surface antigen of Hodgkins and Reed-Sternberg cells using monoclonal antibody Ki-1. The ligand for CD30 is CD30L (CD153). The binding of CD30 to CD30L mediates pleiotropic effects including cell proliferation, activation, differentiation, and apoptotic cell death. CD30 has a critical role in the pathophysiology of Hodgkin's disease and other CD30+ lymphomas. CD30 acts as a costimulatory molecule in thymic negative selection. In addition to its expression on Hodgkin's and Reed-Sternberg cells, CD30 is also found in some non-Hodgkin's lymphomas (including Burkitt's lymphomas), virus-infected T and B cells, and on normal T and B cells after activation. In T cells, CD30 expression is present on a subset of T cells that produce Th2-type cytokines and on CD4+/CD8+ thymocytes that co-express CD45RO and the IL4 receptor. Soluble form of CD30 (sCD30) serves as a marker reflecting Th2 immune response. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. CD30 is a positive regulator of apoptosis, and has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. CD30 is expressed by mononuclear cells in Hodgkin's lymphoma, Reed Sternberg cells and most Anaplastic Large Cell Lymphomas (ALCL). CD30 is also expressed by embryonal carcinomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Gene aliases : CD30, D1S166E, Ki-1, TNFRSF8
Gene ID : (Human) 943
Gene symbol : TNFRSF8
Protein Aliases : CD30, CD30L receptor, Ki-1 antigen, Lymphocyte activation antigen CD30, cytokine receptor CD30, Tumor necrosis factor receptor superfamily member 8, tumor necrosis factor receptor superfamily, member 8
UniProt ID (Human) P28908
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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