Technical guide & protocol Tech Support

Product Specifications

Analytical sensitivity

0.10 ng/mL

Assay range

0.31-20.0 ng/mL

Sample type/volume

Plasma
50 µL
Serum
50 µL
Supernatant
50 µL

Hands-on time

15 min

Time-to-result

3 hr 10 min

Homogenous (no wash)

No

Interassay CV

9.2%

Intraassay CV

7.2%

Instrument

Colorimetric Microplate Reader

Product size

128 Tests

Contents

Pre-coated 96 well Microplate
Detection Antibody
Anti-IgG-HRP
Sample Diluent, lyophilized
Standard (8-well strips x 4)
Wash Buffer Concentrate
Substrate Solution
Sample Diluent
Stop Solution
Lysis Buffer 10x
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

-20°C

Protein name

CD80 (B7-1)

Target sub-specificity

Soluble

Species (tested)

Human

Assay kit format

Instant ELISA Kit

Detector antibody conjugate

HRP

Label or dye

HRP

About This Kit

The Human Soluble CD80 (Hu sCD80) ELISA quantitates Hu sCD80 in human serum, plasma, cell culture supernatants, or other body fluids. The assay will exclusively recognize both natural and recombinant Hu sCD80.

Principle of the method

The Human sCD80 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Target information

CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28, and of an inhibitory receptor CTLA-4 (CD152). Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, and both molecules can also substitute for each other in this process. CD80 is rapidly induced on the surface of in vitro activated B cells, with Epstein Barr Virus (EBV) transformed B cell lines, with Burkitts lymphoma cell lines, with freshly isolated follicular B lymphoma cells, T cells, and monocytes. The B-lymphocyte activation antigen B7-1 provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells. Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Gene aliases : B7, B7-1, B7.1, BB1, CD28LG, CD28LG1, CD80, LAB7

Gene ID : (Human) 941

Gene symbol : CD80

Protein Aliases : Activation B7-1 antigen, BB1, CTLA-4 counter-receptor B7.1, B7, B-lymphocyte activation antigen B7, CD80, CD80 antigen (CD28 antigen ligand 1, B7-1 antigen), costimulatory factor CD80, costimulatory molecule variant IgV-CD80, T-lymphocyte activation antigen CD80

UniProt ID (Human) P33681

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