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Product Specifications
Analytical sensitivity
0.4 ng/mL
Assay range
0.4-100ng/mL
Sample type/volume
Plasma
50 µL
Serum
50 µL
Supernatant
50 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr 45 min
Homogenous (no wash)
No
Interassay CV
<12%
Intraassay CV
<10%
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
Factor VII
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
Human Coagulation Factor VII quantitates human Coagulation Factor VII in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Coagulation Factor VII.
Target information
Coagulation factor VII is a vitamin K-dependent factor essential for hemostasis. This factor circulates in the blood in a zymogen form, and is converted to an active form by either factor IXa, factor Xa, factor XIIa, or thrombin by minor proteolysis. Upon activation of the factor VII, a heavy chain containing a catalytic domain and a light chain containing 2 EGF-like domains are generated, and two chains are held together by a disulfide bond. In the presence of factor III and calcium ions, the activated factor then further activates the coagulation cascade by converting factor IX to factor IXa and/or factor X to factor Xa. Defects in F7 production can cause coagulopathy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : F7, SPCA
Gene ID : (Human) 2155
Gene symbol : F7
Protein Aliases : Coagulation factor VII, coagulation factor VII (serum prothrombin conversion accelerator), eptacog alfa, FVII coagulation protein, Proconvertin, Serum prothrombin conversion accelerator, SPCA
UniProt ID (Human) P08709
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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