Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
The Human Complement Factor D (Adipsin/CFD) ELISA quantitates Hu Complement Factor D in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Complement Factor D.
Principle of the method
The Human Complement Factor D solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Complement factor D is a serine protease of the alternative pathway of complement activation. The protein is a single chain polypeptide with a molecular mass of 25 kDa. Factor D cleaves factor B bound to C3b, generating the alternative pathway C3 convertase C3bBb and releasing the Ba fragment.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Gene aliases : ADIPSIN, ADN, CFD, DF, PFD
Gene ID : (Human) 1675
Gene symbol : CFD
Protein Aliases : Adipsin, C3 convertase activator, Properdin factor D, Complement factor D, complement factor D (adipsin), complement factor D preproprotein, D component of complement (adipsin)
UniProt ID (Human) P00746
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