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Product Specifications
Analytical sensitivity
0.04 ng/mL
Assay range
0.08-5.0 ng/mL
Sample type/volume
Amniotic Fluid
10 µL
Fetal Umbilical Vein Blood
10 µL
Plasma
10 µL
Serum
10 µL
Supernatant
10 µL
Urine
10 µL
Hands-on time
1 hr 20 min
Time-to-result
1 hr 10 min
Homogenous (no wash)
No
Interassay CV
5.8%
Intraassay CV
5.1%
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Sample Diluent
Assay Buffer concentrate
HRP-conjugated Detection Antibody
Wash Buffer
Chromogen
Stop Solution
Controls
Adhesive Plate Covers
Standard
Sample Diluent
Assay Buffer concentrate
HRP-conjugated Detection Antibody
Wash Buffer
Chromogen
Stop Solution
Controls
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
SOD1
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
HRP
Label or dye
HRP
About This Kit
The Human Cu/Zn Superoxide Dismutase(Hu Cu/ZnSOD)) ELISA quantitates Hu Cu/ZnSOD in human serum, plasma, amniotic fluid, urine, fetal umbilical vein blood, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Cu/ZnSOD.
Principle of the method
The Human Cu/ZnSOD solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
SOD1 (superoxide dismutase) binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. This isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Gene aliases : ALS, ALS1, HEL-S-44, homodimer, hSod1, IPOA, SOD, SOD1
Gene ID : (Human) 6647
Gene symbol : SOD1
Protein Aliases : Cu/Zn superoxide dismutase, epididymis secretory protein Li 44, hSod1, indophenoloxidase A, SOD, soluble, Superoxide dismutase 1, Superoxide dismutase [Cu-Zn], superoxide dismutase, cystolic
UniProt ID (Human) P00441
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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