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Product Specifications
Analytical sensitivity
0.08 ng/mL
Assay range
0.08-5 ng/mL
Sample type/volume
Plasma
10 µL
Serum
10 µL
Supernatant
10 µL
Hands-on time
24 hr
Time-to-result
1 hr 10 min
Homogenous (no wash)
No
Instrument
Colorimetric Microplate Reader
Product size
10 x 96 Tests
Contents
Capture (coating) Antibody
Detection Antibody
Standard
Detection Antibody
Standard
Shipping conditions
Wet ice
Storage
-20°C
Protein name
SOD1
Species (tested)
Human
Assay kit format
Matched Antibody Pair
Detector antibody conjugate
HRP
Label or dye
HRP
About This Kit
The Human Cu/ZnSOD Matched Antibody Pair Kit comes with pre-matched antibody pairs and standards to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human Cu/ZnSOD. Buffer reagents and microplates needed to complete the ELISA reaction are sold separately.
Principle of the method
ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Target information
SOD1 (superoxide dismutase) binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. This isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : ALS, ALS1, HEL-S-44, homodimer, hSod1, IPOA, SOD, SOD1
Gene ID : (Human) 6647
Gene symbol : SOD1
Protein Aliases : Cu/Zn superoxide dismutase, epididymis secretory protein Li 44, hSod1, indophenoloxidase A, SOD, soluble, Superoxide dismutase 1, Superoxide dismutase [Cu-Zn], superoxide dismutase, cystolic
UniProt ID (Human) P00441
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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