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Product Specifications
Analytical sensitivity
25 pg/mL
Assay range
27.4-20,000 pg/mL
Sample type/volume
Plasma
50 µL
Serum
50 µL
Supernatant
100 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr 45 min
Homogenous (no wash)
No
Interassay CV
<12%
Intraassay CV
<10%
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
ENC1
Protein family
Chemokines & Receptors
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Human ENC1/CCL28 ELISA quantitates Hu ENC1 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu ENC1.
Principle of the method
The Human ENC1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
The ectoderm-neural cortex-1 (ENC-1) protein is an early and highly specific marker of neural induction in vertebrates. It is a kelch family related protein that functions as an actin-binding protein and has been suggested to be involved in the organization of the actin cytoskeleton during neural fate specification and development of the nervous system. ENC-1 has also been shown to be required for adipocyte differentiation when cytoskeletal reorganization and cell shape change from fibroblastic preadipocytes to spherical adipocytes occur.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : CCL28, ENC-1, ENC1, KLHL35, KLHL37, NRPB, PIG10, TP53I10
Gene ID : (Human) 8507
Gene symbol : ENC1
Protein Aliases : Ectoderm-neural cortex protein 1, ectodermal-neural cortex 1 (with BTB domain), ectodermal-neural cortex 1 (with BTB-like domain), ENC-1, Kelch-like protein 37, Nuclear matrix protein NRP/B, p53-induced gene 10 protein, kelch-like 35, kelch-like family member 37, nuclear restricted protein, BTB domain-like (brain), tumor protein p53 inducible protein 10
UniProt ID (Human) O14682
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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