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Product Specifications
Analytical sensitivity
<1.6 ng/mL
Assay range
1.6-100 ng/mL
Sample type/volume
Cell Lysate
10 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr
Homogenous (no wash)
No
Interassay CV
7.4%
Intraassay CV
4.6%
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Standard Dilution Buffer
Detection Antibody
anti-rabbit-HRP
HRP Diluent
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Standard Dilution Buffer
Detection Antibody
anti-rabbit-HRP
HRP Diluent
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
FAK
Target sub-specificity
Total
Protein subtype
Cytoplasmic Tyrosine Kinases
Protein family
Kinases & Inhibitors
Species (tested)
Human, Mouse, Rat
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
HRP
Label or dye
HRP
About This Kit
The Human FAK (Total) ELISA Kit is a solid-phase sandwich Enzyme-Linked Immunosorbent Assay (ELISA) designed to detect and quantify the level of FAK (Total) in fresh or frozen human, mouse or rat cell lysates. The assay recognizes both natural and recombinant FAK (Total), independent of its phosphorylation state.
Principle of the method
A monoclonal capture antibody specific for FAK (Total) has been coated onto the wells of the 96-well plate. During the first incubation, standards of known content and unknown samples are pipetted into the wells and the antigen binds to the immobilized (capture) antibody. After washing, a rabbit antibody specific for the target protein is added to the wells and serves as a detection antibody by binding to the immobilized protein captured during the first incubation. After washing, a horseradish peroxidase labeled anti-rabbit IgG is added. This binds to the detection antibody to complete the four member sandwich. After a third incubation and washing to remove all the unbound enzyme, a substrate solution (TMB) is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of target protein present in the original specimen and the optical density can be read on a standard microplate reader.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. FAK plays an important role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle. FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, and the binding site has been identified as Tyr925 by site directed mutagenesis. Several transcript variants encoding different isoforms have been found for the FAK gene, but the full-length natures of only three of them have been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Gene aliases : FADK, FAK, FAK1, FRNK, Kiaa4203, mKIAA4203, p125FAK, pp125FAK, PPP1R71, PTK2
Gene ID : (Human) 5747, (Mouse) 14083, (Rat) 25614
Gene symbol : PTK2, Ptk2, Ptk2
Protein Aliases : FADK 1, FAK-related non-kinase polypeptide, Focal adhesion kinase 1, focal adhesion kinase isoform FAK-Del33, Focal adhesion kinase-related nonkinase, focal ashension kinase 1, FRNK, p125FAK, pp125FAK, PPP1R71, Protein phosphatase 1 regulatory subunit 71, protein phosphatase 1, regulatory subunit 71, Protein-tyrosine kinase 2, PTK2 protein tyrosine kinase 2
UniProt ID (Human) Q05397, (Mouse) Q5DTH7, (Rat) O35346
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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