Technical guide & protocol Tech Support

Product Specifications

Analytical sensitivity

0.3 ng/mL

Assay range

0.3-75 ng/mL

Sample type/volume

Supernatant
50 µL
Plasma
50 µL
Serum
50 µL

Hands-on time

1 hr 20 min

Time-to-result

4 hr 45 min

Homogenous (no wash)

No

Interassay CV

<12%

Intraassay CV

<10%

Instrument

Colorimetric Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

2-8°C

Protein name

FGF23

Species (tested)

Human

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

Human FGF-23 quantitates human FGF-23 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human FGF-23.

Target information

The protein encoded by this gene is a member of the fibroblast growth factor family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. The product of this gene inhibits renal tubular phosphate transport. This gene was identified by its mutations associated with autosomal dominant hypophosphatemic rickets, an inherited phosphate wasting disorder. Abnormally high level expression of this gene was found in oncogenic hypophosphatemic osteomalacia, a phenotypically similar disease caused by abnormal phosphate metabolism. Mutations in this gene have also been shown to cause familial tumoral calcinosis with hyperphosphatemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene aliases : ADHR, FGF23, FGFN, HPDR2, HYPF, PHPTC, UNQ3027/PRO9828

Gene ID : (Human) 8074

Gene symbol : FGF23

Protein Aliases : FGF-23, Fibroblast growth factor 23, Phosphatonin, tumor-derived hypophosphatemia inducing factor, Tumor-derived hypophosphatemia-inducing factor

UniProt ID (Human) Q9GZV9

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