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Product Specifications
Analytical sensitivity
85 pg/mL
Assay range
78.13-5000 pg/mL
Sample type/volume
Plasma
2.5 µL
Serum
2.5 µL
Supernatant
100 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr 45 min
Homogenous (no wash)
No
Interassay CV
<12%
Intraassay CV
<10%
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
CD32
Protein subtype
Adaptor Proteins
Protein family
Other Proteins
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Human FcGR2BC (CD32 B/C) ELISA quantitates Hu FcGR2BC in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu FcGR2BC.
Principle of the method
The Human FcGR2BC solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : CD32, CD32A, CD32B, CD32C, CDW32, FCG2, FcGR, FCGR2, FCGR2A, FCGR2A1, FCGR2B, FCGR2C, FCRIIC, IGFR2
Gene ID : (Human) 2212, (Human) 2213, (Human) 9103
Gene symbol : FCGR2A, FCGR2B, FCGR2C
Protein Aliases : CD32, CDw32, Fc fragment of IgG, low affinity II, receptor for (CD32), low affinity IIa, receptor (CD32), low affinity IIb, low affinity IIc, Fc gamma receptor IIa, Fc gamma receptor IIb, Fc gamma receptor IIC, Fc gamma RIIb, Fc-gamma RII-a, Fc-gamma RII-b, Fc-gamma RII-c, Fc-gamma-RIIa, Fc-gamma-RIIb, Fc-gamma-RIIc, fcRII-a, fcRII-b, IgG Fc receptor II-a, IgG Fc receptor II-b, IgG Fc receptor II-c, Immunoglobulin G Fc receptor II, immunoglobulin G Fc receptor II-c, Low affinity immunoglobulin gamma Fc region receptor II-a, Low affinity immunoglobulin gamma Fc region receptor II-b, Low affinity immunoglobulin gamma Fc region receptor II-c
UniProt ID (Human) P12318, (Human) P31994, (Human) P31995
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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