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Product Specifications
Analytical sensitivity
0.25 ng/mL
Assay range
0.25-50 ng/mL
Sample type/volume
Supernatant
50 µL
Plasma
50 µL
Serum
50 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr 45 min
Homogenous (no wash)
No
Interassay CV
<12%
Intraassay CV
<10%
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
Ficolin 3
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
Human Ficolin-3 quantitates human Ficolin-3 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Ficolin-3.
Target information
H-Ficolin (Hakata antigen, Ficolin-3) is an innate immunity pattern recognition molecule found in human serum. H-Ficolin binds to distinct pathogen-associated molecular patterns (PAMP) formed by carbohydrates (GlcNAc, GalNAc and fucose) activating complement through the lectin pathway by means of the associated MASPs ((MBL)-associated serine proteases). H-Ficolin consists of 35 kDa protein chains associated in triplet subunits which are again associated in high molecular weight oligomers by means of disulfide bonds at the N-terminal region.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : FCN3, FCNH, HAKA1
Gene ID : (Human) 8547
Gene symbol : FCN3
Protein Aliases : Collagen/fibrinogen domain-containing lectin 3 p35, Collagen/fibrinogen domain-containing protein 3, Ficolin-3, Hakata antigen
UniProt ID (Human) O75636
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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