Technical guide & protocol Tech Support

Product Specifications

Analytical sensitivity

40 pg/mL

Assay range

40-10,000 pg/mL

Sample type/volume

Supernatant
50 µL
Plasma
50 µL
Serum
50 µL

Hands-on time

1 hr 20 min

Time-to-result

4 hr 45 min

Homogenous (no wash)

No

Interassay CV

<12%

Intraassay CV

<10%

Instrument

Colorimetric Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

2-8°C

Protein name

GLI3

Species (tested)

Human

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

Human GLI-3 quantitates human GLI-3 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human GLI-3.

Target information

This gene encodes a protein which belongs to the C2H2-type zinc finger proteins subclass of the Gli family. They are characterized as DNA-binding transcription factors and are mediators of Sonic hedgehog (Shh) signaling. The protein encoded by this gene localizes in the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. Mutations in this gene have been associated with several diseases, including Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, and postaxial polydactyly types A1 and B.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene aliases : ACLS, GCPS, GLI3, GLI3-190, GLI3FL, PAP-A, PAPA, PAPA1, PAPB, PHS, PPDIV

Gene ID : (Human) 2737

Gene symbol : GLI3

Protein Aliases : GLI-Kruppel family member GLI3, GLI3 form of 190 kDa, GLI3 full-length protein, GLI3-190, GLI3FL, glioma-associated oncogene family zinc finger 3, oncogene GLI3, Transcriptional activator GLI3, zinc finger protein GLI3

UniProt ID (Human) P10071

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