Search Thermo Fisher Scientific
Product Specifications
Analytical sensitivity
20 pg/mL
Assay range
13.72-10,000 pg/mL
Sample type/volume
Plasma
33 µL
Serum
33 µL
Supernatant
100 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr 45 min
Homogenous (no wash)
No
Interassay CV
<12%
Intraassay CV
<10%
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
Galectin 7
Protein family
Other Proteins
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Human Galectin-7 ELISA quantitates Hu Galectin-7 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Galectin-7.
Principle of the method
The Human Galectin-7 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Differential and in situ hybridization studies indicate that this lectin is specifically expressed in keratinocytes and found mainly in stratified squamous epithelium. A duplicate copy of this gene (GeneID:653499) is found adjacent to, but on the opposite strand on chromosome 19.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Gene aliases : Gal-7, GAL7, HKL-14, LGALS7, LGALS7A, LGALS7B, PI7, PIG1
Gene ID : (Human) 3963, (Human) 653499
Gene symbol : LGALS7, LGALS7B
Protein Aliases : Gal-7, Galectin-7, galectin-7B, HKL-14, keratinocyte lectin 14, lectin galactoside-binding soluble 7, lectin, galactoside binding soluble 7, galactoside binding soluble 7B, galactoside-binding, soluble, 7, 7B, p53-induced gene 1 protein, p53-induced protein 1, PI7
UniProt ID (Human) P47929, (Human) P47929
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support