Technical guide & protocol Tech Support

Product Specifications

Analytical sensitivity

0.8 ng/mL

Assay range

0.8-600 ng/mL

Sample type/volume

Supernatant
50 µL
Plasma
50 µL
Serum
50 µL

Hands-on time

1 hr 20 min

Time-to-result

4 hr 45 min

Homogenous (no wash)

No

Interassay CV

<12%

Intraassay CV

<10%

Instrument

Colorimetric Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

2-8°C

Protein name

Glypican 3

Species (tested)

Human

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

Human Glypican 3 quantitates human Glypican 3 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Glypican 3.

Target information

GPC3 is a cell surface proteoglycan that bears heparan sulfate. This protein may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. Members of the glypican-related integral membrane proteoglycan family contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol (GPI) linkage. These proteins may play a role in the control of cell division, growth regulation, and tumor predisposition. Deletion mutations in GPC3 are the cause of Simpson-Golabi-Behmel syndrome (SGBS), also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene aliases : DGSX, GPC3, GTR2-2, MXR7, OCI-5, OCI5, SDYS, SGB, SGBS, SGBS1

Gene ID : (Human) 2719

Gene symbol : GPC3

Protein Aliases : glypican proteoglycan 3, Glypican-3, GTR2-2, heparan sulphate proteoglycan, Intestinal protein OCI-5, MXR7, secreted glypican-3

UniProt ID (Human) P51654

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