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Product Specifications

Analytical sensitivity

5.5 pg/mL

Assay range

63-4,000 pg/mL

Sample type/volume

Plasma
50 µL
Serum
50 µL
Supernatant
50 µL

Hands-on time

15 min

Time-to-result

3 hr 10 min

Homogenous (no wash)

No

Interassay CV

8.3%

Intraassay CV

7.6%

Instrument

Colorimetric Microplate Reader

Product size

128 Tests

Contents

Pre-coated 96 well Microplate
Biotinylated Detection Antibody
SAV-HRP
Sample Diluent, lyophilized
Standard (8-well strips x 4)
Wash Buffer Concentrate
Substrate Solution
Sample Diluent
Stop Solution
Lysis Buffer 10x
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

-20°C

Protein name

HGF

Species (tested)

Human

Assay kit format

Instant ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

The Human Hepatocyte Growth Factor (Hu HGF) ELISA quantitates Hu HGF in human serum, plasma, cell culture supernatants, or other body fluids. The assay will exclusively recognize both natural and recombinant Hu HGF.

Principle of the method

The Human HGF solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Target information

Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Gene aliases : DFNB39, F-TCF, HGF, HGFB, HPTA, SF

Gene ID : (Human) 3082

Gene symbol : HGF

Protein Aliases : fibroblast-derived tumor cytotoxic factor, Hepatocyte growth factor, hepatocyte growth factor (hepapoietin A, scatter factor), Hepatopoietin-A, Scatter factor, SF, lung fibroblast-derived mitogen

UniProt ID (Human) P14210

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