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Product Specifications
Analytical sensitivity
200 pg/mL
Assay range
0.205-50 ng/mL
Sample type/volume
Plasma
50 µL
Serum
50 µL
Supernatant
100 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr 45 min
Homogenous (no wash)
No
Interassay CV
<12%
Intraassay CV
<10%
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
HTRA2
Protein family
Growth Factors & Receptors
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Human HTRA2(OMI) ELISA quantitates Hu OMI in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu OMI.
Principle of the method
The Human OMI solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
The inhibitor of apoptosis (IAP) family of proteins regulates programmed cell death triggered by various stimuli. All IAPs have at least one baculovirus IAP repeat (BIR) motif that is essential for their anti-apoptotic activity (1-3). Recently, a serine protease has been isolated, which is released from the mitochondria upon induction of apoptosis by apoptotic stimuli. This protein called Omi/HtrA2 is a mitochondrial protein that binds to IAP (4). Like Smac/DIABLO, the mature Omi protein contains a conserved IAP-binding motif (AVPS) at its N terminus. The deregulated expression of Omi in the cytoplasm of mammalian cells induces apoptosis indicating that Omi could participate in the mitochondrial apoptotic pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : HTRA2, OMI, PARK13, PRSS25
Gene ID : (Human) 27429
Gene symbol : HTRA2
Protein Aliases : High temperature requirement protein A2, HtrA-like serine protease, HtrA2, Omi stress-regulated endoprotease, protease, serine, 25, Serine protease 25, Serine protease HTRA2, mitochondrial, Serine proteinase OMI
UniProt ID (Human) O43464
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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