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Product Specifications
Analytical sensitivity
50 pg/mL
Assay range
50-4000 pg/mL
Sample type/volume
Supernatant
50 µL
Hands-on time
1 hr 20 min
Time-to-result
3 hr 15 min
Homogenous (no wash)
No
Interassay CV
<8%
Intraassay CV
<8%
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well Microplate
Detection Antibody Concentrate
Anti-IgG-HRP Concentrate
Concentrate Diluent
Standard
Wash Buffer Concentrate
Substrate Solution
Sample Diluent
Stop Solution
Adhesive Plate Covers
Detection Antibody Concentrate
Anti-IgG-HRP Concentrate
Concentrate Diluent
Standard
Wash Buffer Concentrate
Substrate Solution
Sample Diluent
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
IFN beta
Protein family
Cytokines & Receptors
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
HRP
Label or dye
HRP
About This Kit
The Human Interferon beta (Hu IFNβ) ELISA quantitates Hu IFNβ in human cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IFNβ.
Principle of the method
The Human IFNβ solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
Type I Interferons (IFN-alpha/beta) are produced primarily in response to viral infection by Natural IFN-producing cells (IPCs) as part of the host immune response. IFNs can also inhibit the development of tumors. IFN-beta binding results in the activation of the tyrosine kinases Jak1 and Tyk2, phosphorylation of members of the STAT family of transcription factors, and the transcription and expression of the immune response genes. More recently, several members of the toll-like receptor (TLR) family were found to stimulate the production IFN-beta. IFN-beta is currently used clinically for treatment of tumors, infections and multiple sclerosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Gene aliases : IFB, IFF, IFN-beta, IFNB, IFNB1
Gene ID : (Human) 3456
Gene symbol : IFNB1
Protein Aliases : Fibroblast interferon, IFN-beta, Interferon beta, interferon, beta 1, fibroblast, interferon-beta
UniProt ID (Human) P01574
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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