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Product Specifications
Analytical sensitivity
0.03 pg/mL
Assay range
0.39-25.0 pg/ml
Sample type/volume
Plasma
50 µL
Supernatant
50 µL
Serum
50 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr
Homogenous (no wash)
No
Interassay CV
7.5%
Intraassay CV
6.8%
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Pre-coated microwell plate
Biotinylated Detection Antibody
Streptavidin-HRP
Standard
Control
Sample Diluent
Assay Buffer Concentrate
Amplification Reagent I
Amplification Reagent II
Wash Buffer Concentrate
Substrate Solution
Stop Solution
Adhesive Plate Covers
Biotinylated Detection Antibody
Streptavidin-HRP
Standard
Control
Sample Diluent
Assay Buffer Concentrate
Amplification Reagent I
Amplification Reagent II
Wash Buffer Concentrate
Substrate Solution
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
IL-10
Species (tested)
Human
Assay kit format
High Sensitivity Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Human High Sensitivity Interleukin 10 (HS IL-10) ELISA quantitates Hu HS IL-10 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu HS IL-10.
Principle of the method
The Human HS IL-10 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A special amplification reagent step is added to achieve the high sensitivity capability. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : CSIF, GVHDS, IL-10, IL10, IL10A, TGIF
Gene ID : (Human) 3586
Gene symbol : IL10
Protein Aliases : CSIF, Cytokine synthesis inhibitory factor, IL-10, Interleukin-10, T-cell growth inhibitory factor
UniProt ID (Human) P22301
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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