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The Human Interleukin 12 p70 (IL-12 p70) Uncoated ELISA Kit contains pre-matched antibody pairs, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-12 p70. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.
Principle of the method
ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
IL-12 p70, also known as interleukin-12, is a cytokine that plays a critical role in the immune system's response to infections and cancer. It is produced by immune cells, including dendritic cells, macrophages, and B cells, in response to certain pathogens and tumor cells. IL-12 p70 stimulates the production of interferon-gamma and other cytokines, which promote the activation and proliferation of T cells and natural killer cells. IL-12 p70 has been studied as a potential therapeutic target for various diseases and conditions, including infectious diseases, autoimmune disorders, and cancer. It has been shown to enhance immune responses against certain infections and tumors, and to modulate inflammatory responses in autoimmune diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Gene aliases : CLMF, CLMF2, IL-12A, IL-12B, IL12A, IL12B, IMD28, IMD29, NFSK, NKSF, NKSF1, NKSF2, P35
Gene ID : (Human) 3592, (Human) 3593
Gene symbol : IL12A, IL12B
Protein Aliases : CLMF p35, CLMF p40, cytotoxic lymphocyte maturation factor 1, p35, Cytotoxic lymphocyte maturation factor 35 kDa subunit, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL-12 subunit p35, IL-12 subunit p40, IL-12, subunit p35, IL-12A, IL-12B, IL12, subunit p40, IL35 subunit, interleukin 12, p40, interleukin 12A (natural killer cell stimulatory factor 1, p35), interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40), interleukin-12 alpha chain, interleukin-12 beta chain, Interleukin-12 subunit alpha, Interleukin-12 subunit beta, natural killer cell stimulatory factor, 40 kD subunit, NF cell stimulatory factor chain 1, NK cell stimulatory factor chain 1, NK cell stimulatory factor chain 2, NKSF1, NKSF2
UniProt ID (Human) P29459, (Human) P29460
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