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Product Specifications
Analytical sensitivity
0.99 pg/mL
Assay range
3.13-200 pg/mL
Sample type/volume
Plasma
50 µL
Serum
50 µL
Supernatant
50 µL
Hands-on time
15 min
Time-to-result
3 hr 10 min
Homogenous (no wash)
No
Interassay CV
12.6%
Intraassay CV
9%
Instrument
Colorimetric Microplate Reader
Product size
128 Tests
Contents
Pre-coated 96 well Microplate
Biotinylated Detection Antibody
SAV-HRP
Sample Diluent, lyophilized
Standard (8-well strips x 4)
Wash Buffer Concentrate
Substrate Solution
Sample Diluent
Stop Solution
Lysis Buffer 10x
Adhesive Plate Covers
Biotinylated Detection Antibody
SAV-HRP
Sample Diluent, lyophilized
Standard (8-well strips x 4)
Wash Buffer Concentrate
Substrate Solution
Sample Diluent
Stop Solution
Lysis Buffer 10x
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
-20°C
Protein name
IL-13
Species (tested)
Human
Assay kit format
Instant ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Human Interleukin 13 (Hu IL-13) ELISA quantitates Hu IL-13 in human serum, plasma, cell culture supernatants or other body fluids. The assay will exclusively recognize both natural and recombinant Hu IL-13.
Principle of the method
The Human IL-13 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and is involved in regulating inflammatory and immune responses. IL-13 down-regulates macrophage activity, thereby inhibiting the production of pro-inflammatory cytokines and chemokines, and is involved in allergen-induced asthma through mechanisms independent of IgE and eosinophils. IL-13 also regulates several stages of B cell maturation and differentiation, up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. Structurally, IL-13 closely resembles IL-3, IL-4, IL-5 and GM-CSF. The gene encoding IL-13, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with the IL-13 gene particularly close to IL4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : IL-13, IL13, NC30, P600
Gene ID : (Human) 3596
Gene symbol : IL13
Protein Aliases : IL-13, Interleukin-13
UniProt ID (Human) P35225
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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