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Product Specifications
Analytical sensitivity
5 pg/mL
Assay range
4.12-3000 pg/mL
Sample type/volume
Plasma
5 µL
Serum
5 µL
Supernatant
100 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr 45 min
Homogenous (no wash)
No
Interassay CV
<12%
Intraassay CV
<10%
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
IL1R2
Protein family
Cytokines & Receptors
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Human Interleukin 1 Receptor 2 (Hu IL-1R2/IL-1RB) ELISA quantitates Hu IL-1R2 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-1R2.
Principle of the method
The Human IL-1R2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
The protein encoded by this gene is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein binds interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA), and acts as a decoy receptor that inhibits the activity of its ligands. Interleukin 4 (IL4) is reported to antagonize the activity of interleukin 1 by inducing the expression and release of this cytokine. This gene and three other genes form a cytokine receptor gene cluster on chromosome 2q12. Two alternatively spliced transcript variants encoding the same protein have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : CD121b, CDw121b, IL-1R-2, IL-1RT-2, IL-1RT2, IL1R2, IL1R2c, IL1RB
Gene ID : (Human) 7850
Gene symbol : IL1R2
Protein Aliases : antigen CDw121b, CD121 antigen-like family member B, CD121b, CDw121b, IL-1 type II receptor, IL-1R-2, IL-1R-beta, interleukin 1 receptor type II variant 3, interleukin 1 receptor, type II, Interleukin-1 receptor beta, Interleukin-1 receptor type 2, Interleukin-1 receptor type II, type II interleukin-1 receptor, beta
UniProt ID (Human) P27930
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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