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Product Specifications
Analytical sensitivity
31.2 pg/mL
Assay range
31.2-1000 pg/mL
Sample type/volume
Plasma
100 µL
Serum
100 µL
Supernatant
100 µL
Hands-on time
1 hr 30 min
Time-to-result
24 hr
Homogenous (no wash)
No
Instrument
Colorimetric Microplate Reader
Product size
10 x 96 Tests
Contents
Capture (coating) Antibody
Detection Antibody
Standard
Streptavidin-HRP
Detection Antibody
Standard
Streptavidin-HRP
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
IL-2
Species (tested)
Human
Assay kit format
Matched Antibody Pair
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Human IL-2 Antibody Pair Kit comes with pre-matched antibody pairs, standards, and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-2. Buffer reagents needed to complete the ELISA reaction are sold separate as a Buffer Kit for Antibody Pairs (Catalog No. CNB0011). The Assay Buffer included in this Buffer Kit can be used as a blocking reagent for ELISA plates as well as a diluent for ELISA standards and samples, detection antibody, and HRP conjugate.
Principle of the method
ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Target information
Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Gene aliases : IL-2, IL2, lymphokine, TCGF
Gene ID : (Human) 3558
Gene symbol : IL2
Protein Aliases : aldesleukin, IL-2, T-cell growth factor, TCGF, Interleukin-2, involved in regulation of T-cell clonal expansion, T cell growth factor
UniProt ID (Human) P60568
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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