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Product Specifications
Analytical sensitivity
9.38 pg/mL
Assay range
15.63-1000 pg/mL
Sample type/volume
Serum
100 µL
Plasma
100 µL
Hands-on time
1 hr 20 min
Time-to-result
3 hr 30 min
Homogenous (no wash)
No
Interassay CV
<10%
Intraassay CV
<10%
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Shipping conditions
Wet ice
Storage
Refer to product documentation for component specific storage temperature.
Protein name
IL-35
Protein family
IL-12
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Label or dye
HRP
About This Kit
The Human IL-35 ELISA quantitates IL-35 in serum, plasma, and other biological fluids
The Human IL-35 (Interleukin 35) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
IL-35, or Interleukin 35 is a cytokine belonging to the IL12 family, that is produced by regulatory T cells. It is a dimeric protein composed of IL-12-alpha and IL-27-beta chains. Members of this class of cytokines are important in influencing T helper cell differentiation and supressing inflammatory response. IL-35 is known to induce proliferation of regulatory T cells, and reduce the activity of helper Th17 cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : CLMF, IL-12A, IL12A, NFSK, NKSF1, P35
Gene ID : (Human) 3592
Gene symbol : IL12A
Protein Aliases : CLMF p35, cytotoxic lymphocyte maturation factor 1, p35, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12 subunit p35, IL-12, subunit p35, IL-12A, IL35 subunit, interleukin 12, interleukin 12A (natural killer cell stimulatory factor 1, p35), interleukin-12 alpha chain, Interleukin-12 subunit alpha, NF cell stimulatory factor chain 1, NK cell stimulatory factor chain 1, NKSF1
UniProt ID (Human) P29459
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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