Technical guide & protocol Tech Support

Product Specifications

Analytical sensitivity

0.01 ng/mL

Assay range

0.08-5.0 ng/mL

Sample type/volume

Amniotic Fluid
20 µL
Plasma
10 µL
Serum
10 µL
Supernatant
20 µL
Urine
20 µL

Hands-on time

1 hr 20 min

Time-to-result

2 hr 10 min

Homogenous (no wash)

No

Interassay CV

2.2%

Intraassay CV

1.7%

Instrument

Colorimetric Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plates
Standard
Sample Diluent
Assay Buffer concentrate
HRP-conjugated Detection Antibody
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

2-8°C

Protein name

IL-6 Receptor (CD126)

Target sub-specificity

Soluble

Species (tested)

Human

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

HRP

Label or dye

HRP

About This Kit

The Human Interleukin-6 Receptor (Hu IL-6R) ELISA quantitates Hu IL-6R in human serum, plasma, amniotic fluid, urine or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-6R.

Principle of the method

The Human IL-6R solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Target information

Interleukin 6 receptor (IL6R), also known as CD126 (Cluster of Differentiation 126), is a type I cytokine receptor. This gene encodes a subunit of the interleukin 6 (IL6) receptor complex. Interleukin 6 is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in the immune response. The IL6 receptor is a protein complex consisting of this protein and interleukin 6 signal transducer (IL6ST/GP130/IL6-beta), a receptor subunit also shared by many other cytokines. Dysregulated production of IL6 and this receptor are implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer. Alternatively spliced transcript variants encoding distinct isoforms have been reported. A pseudogene of this gene is found on chromosome 9.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Gene aliases : CD126, gp80, IL-6R-1, IL-6RA, IL6Q, IL6R, IL6RA, IL6RQ

Gene ID : (Human) 3570

Gene symbol : IL6R

Protein Aliases : CD126, CD126 antigen, gp80, IL-6 receptor subunit alpha, IL-6R 1, Interleukin-6 receptor subunit alpha, Membrane glycoprotein 80

UniProt ID (Human) P08887

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