Technical guide & protocol Tech Support

Product Specifications

Analytical sensitivity

1.0 pg/mL

Assay range

3.1-200 pg/mL

Sample type/volume

Amniotic Fluid
10 µL
Plasma
10 µL
Serum
10 µL
Supernatant
10 µL
Synovial Fluid
10 µL
Urine
10 µL

Hands-on time

15 min

Time-to-result

3 hr 10 min

Homogenous (no wash)

No

Interassay CV

10.4%

Intraassay CV

6.7%

Instrument

Colorimetric Microplate Reader

Product size

128 Tests

Contents

Pre-coated 96 well Microplate
Biotinylated Detection Antibody
SAV-HRP
Sample Diluent, lyophilized
Standard (8-well strips x 4)
Wash Buffer Concentrate
Substrate Solution
Sample Diluent
Stop Solution
Lysis Buffer 10x
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

-20°C

Protein name

CXCL10 (IP-10)

Species (tested)

Human

Assay kit format

Instant ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

The Human Inflammatory Protein-10 (Hu IP-10/CXCL10) ELISA quantitates Hu sIP-10/CXCL10 in human serum, plasma, cell culture supernatants, urine, amniotic fluid, synovial fluid or other body fluids. The assay will exclusively recognize both natural and recombinant Hu sIP-10/CXCL10.

Principle of the method

The Human sIP-10/CXCL10 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Target information

IP-10 (interferon-gamma induced protein 10 kD; in mouse, also called cytokine responsive gene 2, Crg-2, or mob-1) is a highly inducible, primary response gene that belongs to the CXC chemokine superfamily. It was first cloned in 1985. The biological functions of IP-10 are still unclear. Like Mig, IP-10 has no activity on neutrophils. Its functions include stimulation of monocytes, natural killer and T-cell migration, regulation of T-cell and bone marrow progenitor maturation, modulation of adhesion molecule expression as well as inhibition of angiogenesis. Mig and IP-10 share the same receptor, CXCR3.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Gene aliases : C7, crg-2, CXCL10, gIP-10, IFI10, INP10, IP-10, mob-1, SCYB10

Gene ID : (Human) 3627

Gene symbol : CXCL10

Protein Aliases : 10 kDa interferon gamma-induced protein, Gamma-IP10, IP-10, Small-inducible cytokine B10, C-X-C motif chemokine 10, chemokine (C-X-C motif) ligand 10, gamma IP10, interferon-inducible cytokine IP-10, protein 10 from interferon (gamma)-induced cell line, small inducible cytokine subfamily B (Cys-X-Cys), member 10

UniProt ID (Human) P02778

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