Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
The Human IR (Phospho) [pY1158] ELISA Kit is a solid-phase sandwich Enzyme-Linked Immunosorbent Assay (ELISA) designed to detect and quantify the level of IR (Phospho) [pY1158] in fresh or frozen human cell lysates. Cross-reactivity with mouse and rat cells has been observed. The assay recognizes both natural and recombinant human IR (Phospho) [pY1158].
Principle of the method
A monoclonal capture antibody specific for IR (Phospho) [pY1158] has been coated onto the wells of the 96-well plate. During the first incubation, standards of known content and unknown samples are pipetted into the wells and the antigen binds to the immobilized (capture) antibody. After washing, a rabbit antibody specific for the target protein is added to the wells and serves as a detection antibody by binding to the immobilized protein captured during the first incubation. After washing, a horseradish peroxidase labeled anti-rabbit IgG is added. This binds to the detection antibody to complete the four member sandwich. After a third incubation and washing to remove all the unbound enzyme, a substrate solution (TMB) is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of target protein present in the original specimen and the optical density can be read on a standard microplate reader.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
INSR is a receptor tyrosine kinase. Binding of insulin to the extracellular domain of INSR stimulates glucose uptake. INSR and IGF-1 receptors share major structural and functional similarity. The earliest cellular response to insulin stimulation is autophosphorylation of tyrosine in INSR. In humans, the INSR gene is located on chromosome 19. Defects in INSR are the cause of various insulin resistance syndromes and IGF-1R defects may also cause some forms of growth retardation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Gene aliases : 4932439J01Rik, CD220, D630014A15Rik, HHF5, INSR, IR, IR-A, IR-B
Gene ID : (Human) 3643, (Rat) 24954, (Mouse) 16337
Gene symbol : INSR, Insr, Insr
Protein Aliases : CD220, Insulin receptor, insulin receptor preproprotein, IR
UniProt ID (Human) P06213, (Mouse) P15208
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