Technical guide & protocol Tech Support

Product Specifications

Analytical sensitivity

<0.4 U/mL

Assay range

0.625-40 U/mL

Sample type/volume

Cell Lysate
10 µL

Hands-on time

1 hr 20 min

Time-to-result

4 hr

Homogenous (no wash)

No

Interassay CV

7.6%

Intraassay CV

6%

Instrument

Colorimetric Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate
Standard
Standard Dilution Buffer
Detection Antibody
anti-rabbit-HRP
HRP Diluent
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

2-8°C

Protein name

INSR

Target site-specificity

Phosphorylated Tyr1162, Phosphorylated Tyr1163

Protein subtype

Receptor Tyrosine Kinases

Protein family

Kinases & Inhibitors

Species (tested)

Human, Mouse, Rat

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

HRP

Label or dye

HRP

About This Kit

The Human IR (Phospho) [pY1162/pY1163] ELISA Kit is a solid-phase sandwich Enzyme-Linked Immunosorbent Assay (ELISA) designed to detect and quantify the level of IR (Phospho) [pY1162/pY1163] in fresh or frozen human cell lysates. Cross-reactivity with mouse and rat cells has been observed. The assay recognizes both natural and recombinant Human IR (Phospho) [pY1162/pY1163].

Principle of the method

A monoclonal capture antibody specific for IR (Phospho) [pY1162/pY1163] has been coated onto the wells of the 96-well plate. During the first incubation, standards of known content and unknown samples are pipetted into the wells and the antigen binds to the immobilized (capture) antibody. After washing, a rabbit antibody specific for the target protein is added to the wells and serves as a detection antibody by binding to the immobilized protein captured during the first incubation. After washing, a horseradish peroxidase labeled anti-rabbit IgG is added. This binds to the detection antibody to complete the four member sandwich. After a third incubation and washing to remove all the unbound enzyme, a substrate solution (TMB) is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of target protein present in the original specimen and the optical density can be read on a standard microplate reader.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Target information

INSR is a receptor tyrosine kinase. Binding of insulin to the extracellular domain of INSR stimulates glucose uptake. INSR and IGF-1 receptors share major structural and functional similarity. The earliest cellular response to insulin stimulation is autophosphorylation of tyrosine in INSR. In humans, the INSR gene is located on chromosome 19. Defects in INSR are the cause of various insulin resistance syndromes and IGF-1R defects may also cause some forms of growth retardation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Gene aliases : 4932439J01Rik, CD220, D630014A15Rik, HHF5, INSR, IR, IR-A, IR-B

Gene ID : (Human) 3643, (Rat) 24954, (Mouse) 16337

Gene symbol : INSR, Insr, Insr

Protein Aliases : CD220, Insulin receptor, insulin receptor preproprotein, IR

UniProt ID (Human) P06213, (Mouse) P15208

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