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Human IgG Subclass ELISA Kit
To generate a 6-point standard curve, make serial dilutions of the standard using the Diluent Buffer. When reconstituted in 1 mL, the concentration of the standard is: 13.72 µg⁄mL of IgG1, 5.32 µg⁄mL of IgG2, 1.34 µg⁄mL of IgG3, and 0.76 µg⁄mL of IgG4. Below is the concentration of each IgG when diluted serially in half.
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Product Specifications
Sample type/volume
Serum
10 µL
Hands-on time
20 min
Time-to-result
1 hr 10 min
Homogenous (no wash)
No
Instrument
Colorimetric Microplate Reader
Product size
2 x 96 Tests
Contents
Pre-coated 96 well plates
Standard
Sample Diluent
Assay Buffer concentrate
Serum Control
MAbs to IgG Subclasses(4)
Peroxidase-conjugated Antibody
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Sample Diluent
Assay Buffer concentrate
Serum Control
MAbs to IgG Subclasses(4)
Peroxidase-conjugated Antibody
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
Human IgG
Target sub-specificity
IgG1,IgG2,IgG3,IgG4
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
HRP
Label or dye
HRP
About This Kit
The Human IgG Subclass Profile (Hu IgG) ELISA quantitates Hu IgG subclasses in human serum or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu igG1, IgG2, IgG3 or IgG4.
Principle of the method
The Human IgG ELISAs are designed to measure the amount of immunoglobulin in the sample. Each Immunoglobulin subunit, IgG1, IgG2, IgG3 and IgG4 are added to individual wells and adsorbed to the bottom of the microplate well. Samples, standards, or controls are then added into these wells and bind to the immobilized IgG subunit. Following a wash step, anti-human IgG antibodies conjugated to HRP is added. Following another wash step, a TMB substrate solution is added that reacts with the complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
Human IgG, also known as human immunoglobulin G, is an antibody that is produced by the immune system in response to antigens. It is the most abundant immunoglobulin in the blood and plays a key role in defense against pathogens. IgG works by binding to specific antigens and promoting their clearance from the body through various mechanisms, including phagocytosis and complement activation. IgG is also involved in other immune responses, such as antibody-dependent cellular cytotoxicity and neutralization of toxins. Human IgG can be used in research to study the immune response to pathogens and to develop diagnostic tests and therapeutic interventions for infectious and autoimmune diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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