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Invitrogen
Human IgG4 Uncoated ELISA Kit with Plates
Product Specifications
Analytical sensitivity
1.25 ng/mL
Assay range
1.25-80 ng/mL
Sample type/volume
Plasma
10 µL
Serum
10 µL
Supernatant
10 µL
Hands-on time
1 hr 45 min
Time-to-result
24 hr 30 min
Homogenous (no wash)
No
Instrument
Colorimetric Microplate Reader
Product size
2 x 96 Tests
Contents
Capture Antibody: Pre-titrated, purified antibody
Detection Antibody: Pre-titrated, HRP-conjugated antibody
Standard: Human IgG4 protein for generating standard curve and calibrating samples
10X Coating Buffer: Buffer for plating the Capture Antibody
5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme
Substrate: 1X TMB Solution
Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards
96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
Detection Antibody: Pre-titrated, HRP-conjugated antibody
Standard: Human IgG4 protein for generating standard curve and calibrating samples
10X Coating Buffer: Buffer for plating the Capture Antibody
5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme
Substrate: 1X TMB Solution
Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards
96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
Human IgG4
Species (tested)
Human
Assay kit format
Uncoated ELISA Kit with Plates
Detector antibody conjugate
HRP
Label or dye
HRP
About This Kit
The Human IgG4 Uncoated ELISA Kit contains pre-matched antibody pair, platess and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IgG4. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.
Principle of the method
ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Target information
Human IgG4, also known as human immunoglobulin G4, is a subclass of human IgG antibodies that is produced by the immune system in response to antigens. It is the least abundant subclass of IgG in the blood and plays a minor role in defense against pathogens. IgG4 works by binding to specific antigens and promoting their clearance from the body through various mechanisms, including phagocytosis and complement activation. IgG4 is also involved in other immune responses, such as immune tolerance and suppression of inflammation. Human IgG4 can be used in research to study the immune response to pathogens and to develop diagnostic tests and therapeutic interventions for infectious and autoimmune diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Gene ID : (Human) 3503
Gene symbol : IGHG4
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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