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Product Specifications
Assay range
12.5-500 pg/ml or 156-5000 pg/ml
Sample type/volume
Supernatant
100 µL
Hands-on time
1 hr 20 min
Time-to-result
3 hr 15 min
Homogenous (no wash)
No
Interassay CV
<8%
Intraassay CV
<8%
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well Microplate
Detection Antibody Concentrate
Anti-IgG-HRP Concentrate
Concentrate Diluent
Standard
Wash Buffer Concentrate
Substrate Solution
Sample Diluent
Stop Solution
Adhesive Plate Covers
Detection Antibody Concentrate
Anti-IgG-HRP Concentrate
Concentrate Diluent
Standard
Wash Buffer Concentrate
Substrate Solution
Sample Diluent
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
IFN alpha
Protein family
Cytokines & Receptors
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
HRP
Label or dye
HRP
About This Kit
The Human Interferon alpha A, 2, A/D, D, K and 4b (Hu IFNalphaA, IFNalpha2, IFNalphaA/D, IFNalphaD, IFNalphaK and IFNalpha4b ) ELISA quantitates Hu IFN alpha in human cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IFN alpha.
Principle of the method
The Human IFN alpha solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
IFN alpha (Interferon alpha) is a Type I interferon belonging to a family of structurally related, species-specific proteins exclusive to vertebrates. The interaction of IFN-alpha with its receptor component result in the activation of a number of signaling pathways which are mainly involved in innate immune response against viral infection. In humans, the gene for IFN alpha is located on on chromosome 9. The type I interferons consist of 14 different alpha isoforms (subtypes with slightly different specificities), and single beta, omega, epsilon and kappa isoforms. Homologous molecules are found in many species, including rats and mice (and most mammals), and have been identified in birds, reptiles and fish species. IFN alpha is produced primarily by plasmacytoid dendritic cells, is a potent component of the anti-viral innate immune response and modulates adaptive immunity. Diseases associated with the dysfunction of IFN alpha include Hepatitis C and Hepatitis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Gene aliases : IFL, IFN, IFN-ALPHA, IFN-alphaA, IFN-alphaD, IFNA, IFNA1, IFNA13, IFNA2, IFNA2A, IFNA2B, IFNA2C, IFNA@, INFA2
Gene ID : (Human) 3439, (Human) 3440
Gene symbol : IFNA1, IFNA2
Protein Aliases : alpha-2a interferon, IFN-alpha 1b, IFN-alpha-1/13, Interferon alpha-D, LeIF D, IFN-alpha-2, Interferon alpha-A, LeIF A, interferon alpha 1b, interferon alpha 2a, interferon alpha 2b, interferon alpha A, Interferon alpha-1/13, Interferon alpha-2, interferon, alpha 1, alpha 2, interferon-alpha1
UniProt ID (Human) P01562, (Human) P01563
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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