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Product Specifications
Analytical sensitivity
<0.95 ng/mL
Assay range
0.95-60 ng/mL
Sample type/volume
Cell Lysate
10 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr
Homogenous (no wash)
No
Interassay CV
8.1%
Intraassay CV
5.1%
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Standard Dilution Buffer
Detection Antibody
anti-rabbit-HRP
HRP Diluent
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Standard Dilution Buffer
Detection Antibody
anti-rabbit-HRP
HRP Diluent
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
JAK2
Target sub-specificity
Total
Protein subtype
Cytoplasmic Tyrosine Kinases
Protein family
Kinases & Inhibitors
Species (tested)
Human, Mouse
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
HRP
Label or dye
HRP
About This Kit
The Human JAK2 (Total)) ELISA Kit is a solid-phase sandwich Enzyme-Linked Immunosorbent Assay (ELISA) designed to detect and quantify the level of JAK2 (Total) in fresh or frozen human or mouse cell lysates. The assay recognizes both natural and recombinant human JAK2 (Total), independent of its phosphorylation state.
Principle of the method
A monoclonal capture antibody specific for JAK2 (Total) has been coated onto the wells of the 96-well plate. During the first incubation, standards of known content and unknown samples are pipetted into the wells and the antigen binds to the immobilized (capture) antibody. After washing, a rabbit antibody specific for the target protein is added to the wells and serves as a detection antibody by binding to the immobilized protein captured during the first incubation. After washing, a horseradish peroxidase labeled anti-rabbit IgG is added. This binds to the detection antibody to complete the four member sandwich. After a third incubation and washing to remove all the unbound enzyme, a substrate solution (TMB) is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of target protein present in the original specimen and the optical density can be read on a standard microplate reader.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
Janus kinase 2 (JAK2) is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways and is mutated in polycythemia vera. This kinase contains the catalytic domain (JH1) of JAK2. The activation of JAKs can lead to the phosphorylation of STAT (signal transducers and activatiors of transcription) proteins, which dimerize and translocate to the nucleus. Once translocated to the nucleus, the STAT proteins can modify transcription of numerous genes, including interferon-stimulated genes. JAK2 is required for the IFN gamma-receptor complex initiation and JAK1 functions as an amplifier. Some studies have suggested that the role of JAK2 might be performed by Tyk2 and JAK3, if they were positioned correctly within the IFN gamma-receptor complex. Mutations affecting JAK2 can cause Budd-Chiari syndrome, Polycythemia vera, Thrombocythemia 3, myelofibrosis, or Acute myelogenous leukemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : Fd17, JAK2, JTK10, THCYT3
Gene ID : (Human) 3717, (Mouse) 16452
Gene symbol : JAK2, Jak2
Protein Aliases : JAK-2, Janus kinase 2, Janus kinase 2 (a protein tyrosine kinase), Tyrosine-protein kinase JAK2
UniProt ID (Human) O60674, (Mouse) Q62120
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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