Technical guide & protocol Tech Support

Product Specifications

Analytical sensitivity

0.068 ng/mL

Assay range

0.068-50 ng/mL

Sample type/volume

Supernatant
50 µL
Serum
50 µL
Plasma
50 µL

Hands-on time

1 hr 20 min

Time-to-result

4 hr 45 min

Homogenous (no wash)

No

Interassay CV

<12%

Intraassay CV

<10%

Instrument

Colorimetric Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

2-8°C

Protein name

JAM2

Species (tested)

Human

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

Human JAM-B quantitates human JAM-B in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human JAM-B.

Target information

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is localized in the tight junctions between high endothelial cells. It acts as an adhesive ligand for interacting with a variety of immune cell types and may play a role in lymphocyte homing to secondary lymphoid organs.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene aliases : C21orf43, CD322, JAM-B, JAM2, JAMB, PRO245, UNQ219/PRO245, VE-JAM, VEJAM

Gene ID : (Human) 58494

Gene symbol : JAM2

Protein Aliases : CD322, JAM-2, JAM-B, JAM-IT/VE-JAM, Junctional adhesion molecule 2, Junctional adhesion molecule B, Vascular endothelial junction-associated molecule, VE-JAM

UniProt ID (Human) P57087

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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