Technical guide & protocol Tech Support

Product Specifications

Analytical sensitivity

140 pg/mL

Assay range

156.3-10,000 pg/mL

Sample type/volume

Plasma
50 µL
Serum
50 µL
Supernatant
100 µL

Hands-on time

1 hr 20 min

Time-to-result

4 hr 45 min

Homogenous (no wash)

No

Interassay CV

<12%

Intraassay CV

<10%

Instrument

Colorimetric Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

2-8°C

Protein name

Luteinizing Hormone

Protein family

Other Proteins

Species (tested)

Human

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

The Human Luteinizing Hormone (Hu LH) ELISA quantitates Hu LH in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu LH.

Principle of the method

The Human LH solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Target information

LH is a member of the glycoprotein hormone family, Glycoprotein hormones are heterodimers consisting of a common alpha subunit and an unique beta subunit which confers biological specificity. LH is expressed in the pituitary gland and promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids. The genes for the beta chains of chorionic gonadotropin and for luteinizing hormone are contiguous on chromosome 19q13.3. Mutations in this gene are associated with hypogonadism which is characterized by infertility and pseudohermaphroditism.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Gene aliases : CG-ALPHA, CGA, CGB4, FSHA, GPHa, GPHA1, HCG, HH23, LHA, LHB, LSH-B, LSH-beta, TSHA

Gene ID : (Human) 1081, (Human) 3972

Gene symbol : CGA, LHB

Protein Aliases : Anterior pituitary glycoprotein hormones common subunit alpha, CG-alpha, Choriogonadotropin alpha chain, Chorionic gonadotrophin subunit alpha, chorionic gonadotropin, alpha polypeptide, Follicle-stimulating hormone alpha chain, follicle-stimulating hormone alpha subunit, Follitropin alpha chain, FSH-alpha, Glycoprotein hormones alpha chain, interstitial cell stimulating hormone, beta chain, LH-B, LSH-alpha, Luteinizing hormone alpha chain, luteinizing hormone beta subunit, Luteinizing hormone subunit beta, Lutropin alpha chain, Lutropin beta chain, LSH-B, LSH-beta, Lutropin subunit beta, Thyroid-stimulating hormone alpha chain, Thyrotropin alpha chain, TSH-alpha

UniProt ID (Human) P01215, (Human) P01229

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