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Product Specifications
Analytical sensitivity
6 pg/mL
Assay range
0-40 ng/ml
Sample type/volume
Serum
10 µL
Plasma
10 µL
Hands-on time
1 hr 20 min
Time-to-result
3 hr 15 min
Homogenous (no wash)
No
Interassay CV
<10%
Intraassay CV
<6%
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate, Standard (2 vials), HRP Detection Antibody, Assay buffer Concentrate, Wash buffer concentrate, TMB, Stop solution, Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
MBL2
Protein family
Lectin
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
HRP
Label or dye
HRP
About This Kit
The Human Mannose Binding Lectin (Hu MBL-2) ELISA quantitates Hu MBL-2 in human serum or plasma. The assay will exclusively recognize both natural and recombinant Hu MBL-2.
Principle of the method
The Human MBL-2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
MBL2 is a calcium-dependent lectin involved in innate immune defense. It binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. It also binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. MBL2 may bind DNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : COLEC1, HSMBPC, MBL, MBL2, MBL2D, MBP, MBP-C, MBP1, MBPD
Gene ID : (Human) 4153
Gene symbol : MBL2
Protein Aliases : Collectin-1, mannan-binding lectin, Mannan-binding protein, Mannose-binding lectin, mannose-binding lectin (protein C) 2, soluble (opsonic defect), mannose-binding lectin 2, Mannose-binding protein C, MBL2, MBP-C, MBP1
UniProt ID (Human) P11226
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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