Technical guide & protocol Tech Support

Product Specifications

Analytical sensitivity

0.1 ng/mL

Assay range

0.1-25 ng/mL

Sample type/volume

Supernatant
50 µL
Serum
50 µL
Plasma
50 µL

Hands-on time

1 hr 20 min

Time-to-result

4 hr 45 min

Homogenous (no wash)

No

Interassay CV

<12%

Intraassay CV

<10%

Instrument

Colorimetric Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

2-8°C

Protein name

MFGE8 (Lactadherin)

Species (tested)

Human

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

Human MFG-E8 quantitates human MFG-E8 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human MFG-E8.

Target information

This gene encodes a preproprotein that is proteolytically processed to form multiple protein products. The major encoded protein product, lactadherin, is a membrane glycoprotein that promotes phagocytosis of apoptotic cells. This protein has also been implicated in wound healing, autoimmune disease, and cancer. Lactadherin can be further processed to form a smaller cleavage product, medin, which comprises the major protein component of aortic medial amyloid (AMA). Alternative splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene aliases : BA46, EDIL1, HMFG, hP47, HsT19888, MFG-E8, MFGE8, MFGM, OAcGD3S, SED1, SPAG10

Gene ID : (Human) 4240

Gene symbol : MFGE8

Protein Aliases : Breast epithelial antigen BA46, HMFG, Lactadherin, medin, MFG-E8, MFGM, Milk fat globule-EGF factor 8, O-acetyl disialoganglioside synthase, SED1, sperm associated antigen 10, sperm surface protein hP47

UniProt ID (Human) Q08431

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