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Product Specifications
Analytical sensitivity
2 pg/mL
Assay range
1.37-1000 pg/mL
Sample type/volume
Plasma
50 µL
Serum
50 µL
Supernatant
100 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr 45 min
Homogenous (no wash)
No
Interassay CV
<12%
Intraassay CV
<10%
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
CXCL1 (GRO alpha)
Protein family
GPCR (G-Protein Coupled Receptors)
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Human MGSA alpha/CXCL1 ELISA quantitates Hu MGSAα in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu MGSAα.
Principle of the method
The Human MGSAα solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
CXCL1, CXCL2, and CXCL3, also known respectively as GRO alpha, GRO beta (MIP-2 alpha) and GRO gamma (MIP-2 beta), are members of the CXC subfamily of chemokines. Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Gene aliases : CXCL1, FSP, GRO, GRO1, GROA, MGSA, MGSA-a, NAP-3, SCYB1
Gene ID : (Human) 2919
Gene symbol : CXCL1
Protein Aliases : C-X-C motif chemokine 1, chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha), fibroblast secretory protein, GRO-alpha(1-73), GRO1 oncogene (melanoma growth stimulating activity, GRO1 oncogene (melanoma growth-stimulating activity), Growth-regulated alpha protein, melanoma growth stimulating activity, alpha, Melanoma growth stimulatory activity, melanoma growth stimulatory activity alpha, MGSA, MGSA alpha, NAP-3, Neutrophil-activating protein 3
UniProt ID (Human) P09341
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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