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Product Specifications
Analytical sensitivity
3 pg/mL
Assay range
4.1-1000 pg/mL
Sample type/volume
Plasma
3.3 µL
Serum
3.3 µL
Supernatant
100 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr 45 min
Homogenous (no wash)
No
Interassay CV
<12%
Intraassay CV
<10%
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
MIP-1 delta
Protein family
Chemokines & Receptors
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Human Macrophage Inflammatory Protein-1 delta/CCL15 (MIP-1δ) ELISA quantitates Hu MIP-1δ in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu MIP-1δ.
Principle of the method
The Human MIP-1δ solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
This gene is located in a cluster of similar genes in the same region of chromosome 17. These genes encode CC cytokines, which are secreted proteins characterized by two adjacent cysteines. The product of this gene is chemotactic for T cells and monocytes, and acts through C-C chemokine receptor type 1 (CCR1). The proprotein is further processed into numerous smaller functional peptides. Naturally-occurring readthrough transcripts occur from this gene into the downstream gene, CCL14 (chemokine (C-C motif) ligand 14).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : CCL15, HCC-2, HMRP-2B, LKN-1, LKN1, MIP-1 delta, MIP-1D, MIP-5, MIP5, MRP-2B, NCC-3, NCC3, SCYA15, SCYL3, SY15
Gene ID : (Human) 6359
Gene symbol : CCL15
Protein Aliases : C-C motif chemokine 15, chemokine (C-C motif) ligand 15, Chemokine CC-2, HCC-2, leukotactin 1, Leukotactin-1, LKN-1, Macrophage inflammatory protein 5, MIP-1 delta, MIP-5, Mrp-2b, NCC-3, new CC chemokine 3, small inducible cytokine subfamily A (Cys-Cys), member 15, Small-inducible cytokine A15
UniProt ID (Human) Q16663
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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