Technical guide & protocol Tech Support

Product Specifications

Analytical sensitivity

0.15 ng/mL

Assay range

0.15-100 ng/mL

Sample type/volume

Supernatant
50 µL

Hands-on time

1 hr 20 min

Time-to-result

4 hr 45 min

Homogenous (no wash)

No

Interassay CV

<12%

Intraassay CV

<10%

Instrument

Colorimetric Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

2-8°C

Protein name

MMP7

Species (tested)

Human

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

Human MMP-7 quantitates human MMP-7 in supernatant. The assay will exclusively recognize both natural and recombinant human MMP-7.

Target information

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades proteoglycans, fibronectin, elastin and casein and differs from most MMP family members in that it lacks a conserved C-terminal protein domain. The enzyme is involved in wound healing, and studies in mice suggest that it regulates the activity of defensins in intestinal mucosa. The gene is part of a cluster of MMP genes which localize to chromosome 11q22. 3.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene aliases : MMP-7, MMP7, MPSL1, PUMP-1, PUMP1

Gene ID : (Human) 4316

Gene symbol : MMP7

Protein Aliases : Matrilysin, Matrin, matrix metallopeptidase 7 (matrilysin, uterine), matrix metalloproteinase 7 (matrilysin, Matrix metalloproteinase-7, MMP-7, Pump-1 protease, uterine matrilysin, Uterine metalloproteinase

UniProt ID (Human) P09237

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