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Product Specifications
Analytical sensitivity
0.38 ng/mL
Assay range
0.63-40 ng/mL
Sample type/volume
Serum
100 µL
Plasma
100 µL
Hands-on time
1 hr 20 min
Time-to-result
3 hr 30 min
Homogenous (no wash)
No
Interassay CV
<10%
Intraassay CV
<10%
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Shipping conditions
Wet ice
Storage
Refer to product documentation for component specific storage temperature.
Protein name
MUC5B
Protein family
Mucin
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Label or dye
HRP
About This Kit
The Human Mucin 5 Subtype B (MUC5B) ELISA quantitates MUC5B in serum, plasma, and other biological fluids
The Human Mucin 5 Subtype B (MUC5B) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
Mucins (MUCs) are heavily glycosylated glycoproteins that are synthesized either as membrane-bound or secreted proteins. Mucins are noteworthy because they have an aberrant expression profile in various malignancies. Mucins normally play a protective role for epithelial tissues and are expressed in the gastrointestinal, respiratory, and genito-urinary tracts. Mucins may also be involved in the renewal and differentiation of the epithelium, and the modulation of cell adhesion and cell signaling. MUC5B is thought to play a vital role in maintaining oral health, and may also be involved in respiratory and oral disease.2 MUC5B expression has been studied in gastric carcinoma as well as chronic obstructive pulmonary disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : MG1, MUC-5B, MUC5, MUC5B, MUC9
Gene ID : (Human) 727897
Gene symbol : MUC5B
Protein Aliases : Cervical mucin, cervical mucin MUC5B, High molecular weight salivary mucin MG1, MUC-5B, mucin 5, subtype B, tracheobronchial, Mucin-5 subtype B, Mucin-5B, Sublingual gland mucin
UniProt ID (Human) Q9HC84
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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