Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
The Human Phosphorylated Neurofilament H ELISA Kit (pNF-H) ELISA quantitates pNF H in serum, plasma and other biological fluids.
Principle of the method
The Human Phosphorylated Neurofilament H (pNF H) solid phase sandwich ELISA (enzyme linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target specific antibody has been pre coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-H has an important role in mature axons and is involved in amyotrophic lateral sclerosis (ALS).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Gene aliases : CMT2CC, KIAA0845, NEFH, NFH
Gene ID : (Human) 4744
Gene symbol : NEFH
Protein Aliases : 200 kDa neurofilament protein, Neurofilament heavy polypeptide, Neurofilament triplet H protein, neurofilament, heavy polypeptide 200kDa, NF-H
UniProt ID (Human) P12036
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