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![Human NF-H (Phospho) [pS61/pS76] ELISA Kit (EEL191)](https://tfcom-global-nginx.commerceprod.thermofisher.com/antibody/images/650/Sandwich-Direct-ELISA-Kit-Packaging-650x600.jpg?time=20220826 "Human NF-H (Phospho) [pS61/pS76] ELISA Kit (EEL191)")
![Human NF-H (Phospho) [pS61/pS76] ELISA Kit (EEL191)](https://tfcom-global-nginx.commerceprod.thermofisher.com/antibody/images/150/Sandwich-Direct-ELISA-Kit-Packaging-650x600.jpg?time=20220826)
Product Specifications
Analytical sensitivity
46.88 pg/mL
Assay range
78.13-5000 pg/mL
Sample type/volume
Tissue Homogenate
100 µL
Plasma
100 µL
Serum
100 µL
Cell Lysate
100 µL
Hands-on time
1 hr 20 min
Time-to-result
2 hr 30 min
Homogenous (no wash)
No
Interassay CV
<10%
Intraassay CV
<10%
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Shipping conditions
Wet ice
Storage
Refer to product documentation for component specific storage temperature.
Protein name
NF-H
Species (reported)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
Unconjugated
About This Kit
The Human Phosphorylated Neurofilament H ELISA Kit (pNF-H) ELISA quantitates pNF H in serum, plasma and other biological fluids.
Principle of the method
The Human Phosphorylated Neurofilament H (pNF H) solid phase sandwich ELISA (enzyme linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target specific antibody has been pre coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Target information
Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-H has an important role in mature axons and is involved in amyotrophic lateral sclerosis (ALS).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : CMT2CC, KIAA0845, NEFH, NFH
Gene ID : (Human) 4744
Gene symbol : NEFH
Protein Aliases : 200 kDa neurofilament protein, Neurofilament heavy polypeptide, Neurofilament triplet H protein, neurofilament, heavy polypeptide 200kDa, NF-H
UniProt ID (Human) P12036
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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