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Product Specifications

Analytical sensitivity

0.05 ng/mL

Assay range

0.05-10 ng/mL

Sample type/volume

Supernatant
50 µL
Serum
50 µL
Plasma
50 µL

Hands-on time

1 hr 20 min

Time-to-result

4 hr 45 min

Homogenous (no wash)

No

Interassay CV

<12%

Intraassay CV

<10%

Instrument

Colorimetric Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

2-8°C

Protein name

NOTCH3

Species (tested)

Human

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

Human Notch-3 quantitates human Notch-3 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Notch-3.

Target information

NOTCH3 is the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction (with its cell-bound ligands delta, serrate) establishes an intercellular signalling pathway that plays a key role in neural development. Homologues of the notch-ligands have also been identified in human. NOTCH3 functions as a receptor for membrane-bound ligands Jagged1, Jagged2, and Delta1 to regulate cell-fate determination. Mutations in NOTCH3 have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene aliases : CADASIL, CADASIL1, CASIL, IMF2, LMNS, NOTCH3

Gene ID : (Human) 4854

Gene symbol : NOTCH3

Protein Aliases : Neurogenic locus notch homolog protein 3, Notch 3, Notch homolog 3

UniProt ID (Human) Q9UM47

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